Chromatography Techniques and Spectrometry

Raman Spectroscopy

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Raman spectroscopy provides the information on molecular vibrations and crystal structures, which is one of the vibrational spectroscopic techniques used in Spectroscopy. To irradiate a sample, this technique uses a laser light source and generates an imperceptible amount of Raman scattered light, which is detected using a CCD camera as a Raman spectrum. In a Raman spectrum the characteristic fingerprinting pattern makes it possible to identify substances including polymorphs and evaluate local crystallinity, orientation and stress.

Some unique advantages of Raman Spectroscopy:

• Non-contact and non-destructive analysis
• High spatial resolution can be measured up to sub-micron scale
• Using a confocal optical system, In-depth analysis of transparent samples can be done
• Sample preparation is not needed
• Measuring of both organic and inorganic substances can be done.
• Measurement of samples in various states such as gas, liquid, solution, solid, crystal, emulsion can be done.
• Through a glass window samples in a chamber can be measured
• Typically, only 10 m.sec to 1 sec exposure to get a Raman spectrum
• Imaging analysis is possible by scanning the motorized stage or laser beam

Raman spectroscopy plays an effective role in departments of R&D, QA and QC in a variety of industrial and academic fields such as semiconductors, polymers, pharmaceuticals, batteries, life sciences and many other departments.