Chromatography Techniques and Spectrometry

Recent Advances in Chromatography-HPLC

The HPLC methodology applicable for the analysis of biological samples makes it possible for the identification of many metabolites. Samples collect from two human embryos culture medium were analysis by high-pressure liquid chromatography–mass spectrometry (HPLC–MS). They work based on the principle that many microorganisms have their own unique mass spectral signature based on the particular proteins and peptides that are present in the cells. Identification of unknown peaks in gas chromatography (GC-MS)-technique based discovery metabolomics is challenging, and remains necessary to permit discovery of novel or unexpected metabolites that may allergic diseases processes and further our understanding of how genotypes related to phenotypes.