Chromatography Techniques and Spectrometry

Chromatography-HPLC as Separation Techniques

High Performance Liquid Chromatography (HPLC) technique is a non-destructive procedure for resolving a complex mixture into its individual fractions or compounds. It is based on the differential migration of solutes with the solvents. The solutes in a mobile phase go through stationary phase. Those solutes with a high affinity for the mobile phase will spend more times in this phase than the solutes that prefer the stationary phase. As the solute rise up through the stationary phase and they separate. The process is called chromatographic development. The fraction with greater affinity to stationary layer travels slower and shorter distance while that with less affinity travels faster and longer distance.

The following techniques are used for separation: