Chromatography-HPLC as Separation Techniques
High Performance Liquid Chromatography (HPLC) technique is a non-destructive procedure for resolving a complex mixture into its individual fractions or compounds. It is based on the differential migration of solutes with the solvents. The solutes in a mobile phase go through stationary phase. Those solutes with a high affinity for the mobile phase will spend more times in this phase than the solutes that prefer the stationary phase. As the solute rise up through the stationary phase and they separate. The process is called chromatographic development. The fraction with greater affinity to stationary layer travels slower and shorter distance while that with less affinity travels faster and longer distance.
The following techniques are used for separation:
- Flash Column Chromatography
- Ion exchange chromatography
- Affinity chromatography
- Size-exclusion chromatography
Related Conference of Chromatography-HPLC as Separation Techniques
March 13-14, 2025
2nd International Conference on Drug Discovery and Development
Prague, Czech Republic
April 07-08, 2025
17th International Conference & Expo on Chromatography Techniques
Vancouver, Canada
June 25-26, 2025
15th World Congress on Analytical & Bioanalytical Techniques
Aix-en-Provence, France
September 25-26, 2025
25th International Conference on Medicinal Chemistry and Drug Design
Zurich, Switzerland
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