Hyphenated Chromatography Methods


The biophysical hyphenated technique is developed from the coupling of a separation technique and an on-line spectroscopy detection technology. Several remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural product, pre-isolation analyses of crude purification or fraction from various natural sources, isolation and detection of natural products, chemical fingerprinting, testing of herbal products, de-replication of natural products, and metabolomics.



Isolation and purification can be achieved by various modern hyphenated methods



 


  • Nuclear Magnetic Resonance (NMR)
  • Electrospray ionization tandem mass spectrometry (ESI-MS-MS)
  • Electrospray ionization tandem mass spectrometry (ESI-MS-MS)
  • HPLC-ESI-MS

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